NEW ! ! ! PATTERN STIMULATION FOR OPTOGENETICS INTEGRATED WITH FUNCTIONAL IMAGING

MULTI-PURPOSE TURNKEY BRAIN IMAGERS – THE 3001 FAMILY

  • VSD optical imaging of cortical functional architecture in vivo or in vitro.
  • VSD optical imaging of network dynamics in vivo or in vitro.
  • Intrinsic optical imaging of cortical functional architecture in vivo.
  • Intrinsic optical imaging for delineation of functional borders during neurosurgery.
  • Intrinsic optical imaging of activity dependent signals in vitro.
  • On-line visualization of changes in cerebral blood volume and flow.
  • Imaging oxygen saturation in vivo and in vitro.
  • Calcium dye imaging of brain slices and other in vitro. preparations.
  • Potassium and sodium imaging.
  • Imaging of intrinsic metabolic markers, cytochromes, NADH, flavins, etc.
  • Imaging of other extrinsic absorption or fluorescent probes for small ligands and proteins.

CHECK THE NEW PRODUCTS IN THE 3001 FAMILY !

Brain Imager Celox

CELOX : Our Fastest Camera Ever, from ISI Speeds to 10,000 Hz VSD Imaging!

LYNX: Our Ultra Low Light Level Camera for Flavo-Protein & Calcium Imaging!

 

 

  • Unsurpassed for VSD imaging at rates up to 1 kHz – in-vivo or in-vitro
  • Many camera options, for a very wide choice of speeds and binning options.
  • Lab interface with 24 BNC connections to your other lab equipment, for total flexibility

MULTIPLE IMAGING SYSTEMS

  • Fastest VSD Imaging: 1000Hz at 544×244 pixels.
  • Best for ISI: 1312 x 1082 pixels at 108 Hz.

pdf iconImager 3001-Celox

  • VSD Imaging at 100Hz at 512×512.
  • ISI at 60 Hz at 1024×1024.
  • Optimal for Medium Light Level Applications
    • Ca imaging at the Population level
    • Auto fluorescence

pdf iconImager 3001/M

  • Low Noise CCD Camera – High Sensitivity and High Well Depth .
  • Intrinsic Optical Imaging of Cortical Functional Architecture.

pdf iconImager 3001/S

  • Effective for Targeted 2-Photon Imaging Experiment.
  • Targeted Electrode Recording, Stimulation or Tracing Injections.

pdf iconImager 3001/C

  • Imaging GCamP6 or Organic Calcium Probe.
  • Flavo-Proteins Fluorescence Signals for Intrinsic Optical Imaging
    of Cortical Functional Architecture in vivo.

pdf iconImager 3001-Lynx

  • Optogenetics-Photo Stimulation with OptoImage Pattern
    Stimulation & Functional Optical Imaging.

pdf iconOptoImage

 

OVERVIEW

The OI Imager 3001 image data acquisition and analysis system carries out optical imaging based on both intrinsic optical signal and voltage sensitive dye (VSD) signals-as well as on signals from calcium dyes and other extrinsic optical probes. The Imager 3001 can monitor blood volume and flow changes, as well as arterial pulsation and the respiratory motion of cortex. Explorations of cortical microcirculation with the Imager 3001 may also help improve the spatial resolution and interpretation of functional MRI and PET imaging on human subjects, or even lead to MRI imaging based on activity-dependent changes in new physiological parameters.

The Imager 3001 is not a bare-bone camera-and-frame grabber combination, leaving the user to figure out the means of adapting it to experimental use. Rather, OI offers and supports it as an integrated solution specifically designed for intrinsic optical signal, VSD, and other types of neuronal optical imaging. OI also offers many imaging accessories for use with the Imager 3001, and an on-site installation program to help you get your imaging experiments up and running.

Not only do the imaging functions of the Imager 3001 complement each other, they can also complement non-imaging techniques that may already be used in your lab. Imaged maps provide information about functional context that can be used to guide site selection for electrode recordings (intracellular or extracellular), or to target micro-stimulation, tracer injections, or other experimental manipulations. Designed from the experience of working scientists, the Imager 3001’s laboratory interface can communicate with much of the standard equipment found in neurophysiology and biophysics laboratories.

 

Intrinsic Optical Signals
As an intrinsic signal imager, the Imager 3001 system detects tiny intrinsic changes in the optical properties of electrically and/or metabolically active brain tissue (signals as small as 1 part in 10,000 are detectable with signal averaging).

The pioneering work of Hill, Keynes, Chance, Jobsis and their colleagues first demonstrated the existence of these intrinsic signals nearly fifty years ago. However, since these signals are very small, their use for imaging of the functional architecture of cortex began only in 1986, as technology and techniques were improved. One source for the activity-dependent optical signal is a small change in the color of the tissue produced by changes in oxygen delivery from oxy-hemoglobin within the capillaries in response to metabolic demand. Other intrinsic signals originate from activity-dependent light scattering changes, and changes in the oxidation states of intrinsic chromophores such as cytochromes.

Intrinsic imaging has facilitated high-resolution imaging of the adult functional architecture of the cerebral cortex in the living brain of mice, rats, guinea pigs, gerbils, ferrets, cats, monkeys and humans. In some animals, activity maps have also been obtained through intact dura and thinned bone, which allows visualization of the development of the functional architecture of the cortex over long periods of time. Success has also been achieved with chronic recording paradigms in larger and adult mammals. Activity-dependent intrinsic signals have also facilitated in-vitro studies in brain slices, and in the isolated but intact mammalian brain.

Voltage Sensitive Dye
The term “voltage sensitive dye” (VSD) is used for compounds that act as optical transducers of membrane potential changes. These probes are used to stain a living preparation. Applied to the brain, they bind to the external surface of the membranes of living cells without interrupting their normal function. Once introduced into a preparation, VSDs rapidly (within a microsecond) alter the intensity and/or wavelengths of fluorescent light they emit as a function of changes in neuronal membrane potential.
Recent improvements in the VSD probes available for in vivo VSD imaging (see the Voltage Sensitive Dyes product sheet for more information) have dramatically increased the signal-to noise-level obtainable-by as much as 10-30 times over previous probes. Combined with the Imager 3001, in vivo or in vitro, voltage sensitive dyes can now provide both fast time resolution and the spatial resolution required to visualize rapid, complex spatio-temporal patterns of neuronal activity.

Calcium Dyes and Other Optical Probes
Calcium signals are usually more than an order of magnitude larger than the VSD signal. Due to this fact, the Imager 3001/M New back thinned 100% fill factor high-resolution camera performs well in detecting small calcium signals, although it is optimized for high light level applications.
See OI’s technical white papers for more information on all these imaging techniques

Imager 3001: A Multi-Purpose Approach

The Imager 3001’s multi-purpose design is a powerful approach to optical imaging. The intrinsic optical signal and VSD imaging techniques each excel at revealing different information about the brain’s functional architecture. Intrinsic optical signal imaging is particularly suited to revealing high-resolution spatial features of the brain’s functional architecture.

VSD imaging excels at revealing the temporal structure of neuronal responses across regions of exposed brain. And calcium imaging extends the power of the system further. Irrespective of the signal used to extract physiological information, the Imager 3001 is well suited to study nearly all the preparations used in brain research explorations.

All Imager 3001 models include the following:

  • VDAQ 2.6
    The latest version of our time-tested data acquisition system running under Windows XP?. It allows for a wide variety of experimental paradigms, live image display for focusing, live display of enhanced differential images, feedback during experiments with flexible on-line analysis displays, and an exceptionally intuitive user interface.
  • WinMix 2.0
    The latest version of our flexible data analysis software, and easy enough to use that it can provide critical information while the experiment is still in progress. WinMix includes utilities to convert or view VDAQ data files or movies, a scripting component, easy-to-use interactive image processing tools, and various export capabilities that let you transport your data or images to 3rd party software.
  • Imaging Camera
    Camera type varies by model, details are provided in our product sheets.
  • Laboratory Interface Unit
    All Imager 3001 systems include a laboratory interface panel that allows you to connect the imager to standard experimental apparatuses such as stimulators, shutters, respirators, etc. All systems also include an “artificial cortex” target for calibration and testing. The BNC Panel allows you to connect your data acquisition system to standard laboratory equipment.
  • An optional Analysis Workstation computer, so that you can perform fast WinMix data analysis while continuing to collect data.
  • All systems are delivered in rugged rack-mount chassis with heavy-duty power supplies and late model motherboards, generous amounts of RAM and disk space, high-speed CPUs, and roomy RAID0 disk arrays for fast data access.
  • All models are turn-key systems. They arrive with all the necessary cables and software, with all software installed and tested. Installation of the cables is shown in an illustrated Cable Installation Guide, so you can be up and running in a very short time.
  • Systems containing both an Acquisition Workstation and an Analysis Workstation include a network hub to interconnect the two computers.
  • ADAQ Option
    ADAQ, an integrated Analog Data AcQuisition facility for VDAQ, lets you make electrical recordings that are co-triggered with your image recording. You can collect up to 16 channels of electrical activity (or 8 differential channels) at speeds of up to 500,000 samples/second, with 12-bit resolution. ADAQ data files are similar in format to existing VDAQ block data files, and we will offer enhanced versions of our analysis tools that can work with analog waveform data.See our Accessories product sheets for imaging items sold separately.
    Monitors included by special order only.

To learn more about current Imager 3001 models, see our product sheets in PDF formats:

VDAQ 2.6 (Video Data AcQuisition) is the next-generation data acquisition component of the Imager 3001, running under Windows XP?. VDAQ runs intrinsic and voltage sensitive dye imaging experiments as well as calcium dye and other extrinsic probe experiments.

It controls image acquisition, as well as external devices such as a stimulator, respirator, and illumination shutter. All experimental parameters can be saved to disk, to allow easily restoring experimental settings from session to session.

Other external hardware devices can also be controlled and monitored as required according to the experiment. Alternatively, VDAQ can itself be controlled through a serial port connection for awake behaving monkey experiments.

VDAQ also has functions for performing the setup tasks that are performed at the beginning of an experimental imaging session. It provides on-line image analysis functions that are critical to monitoring and evaluating the progress and quality of experiments as they unfold. Finally, VDAQ contains the functions used to calibrate and test the video, optical, and image processing components of the instrument.

 

 

LongDaq collects brain imaging data continuously, limited only by the speed and free space of the available storage. It’s available as either an add-on to a Vdaq system, or as a system in its own right.
LongDaq, Optical Imaging’s cost-effective continuous imaging software, now supports a stim-map feature that provides fine-grained control of stimulus output bits during a continuous imaging session. This provides useful synchronization information and lets LongDaq trigger stimulators and other devices during acquisition.
LongDaq‘s useful Quality Assurance data is now saved automatically each time data is collected.
Both Vdaq and LongDaq now allow for stimulus input information to be provided via UDP network packets. Previously, such information could only be provided using TTL level inputs.

OptImage- High End MatLab Based Analysis

WinMix’s interactive tools include:

  • Viewers for image and data files.
  • An image-processing component for filtering, image histograms, line profiles, image arithmetic
  • A polar analysis module for producing polar plots from groups of image files
  • A MovieLoop facility for comparing pairs of images, or working with movies of data
  • Image conversion to a variety of popular image formats, including BMP, TIF, and JPEG

ACCESSORIES

In addition to its Imager 3001 systems, OI sells a range of optical, mechanical, and electrical accessories to help get your brain imaging research efforts quickly under way. These include custom-designed camera mounts, cranium sealing chambers, and illumination and imaging optics. Specifications are subject to change without prior notice.

OI‘s powered tabletop camera mount offers complete freedom for camera positioning. The three long-travel axes are controlled manually, or from a digital panel which can record and return to a set position with micron precision.

The camera holding assembly can be tilted, rotated, and focused by translation from the adjustable camera holder itself. Electronic control of the translation focus is available by special order.

Base
90×100 cm
Max. Height
135 cm
Degrees of Freedom
6

Robotic camera mount

(isolation table not included; see below for installation dimensions)

 

Camera mount control panel

OI‘s cranium sealing chambers protect and re-pressurize exposed brain after trepanning. Ports for sterile fluid exchange allow chronic maintenance of subject animals. Two inner diameters are available: 20 mm titanium (middle row), and 15 mm titanium (bottom). Chambers ship with single gasket and coverslip; replacements available in lots of 20.

 

Macroscope for fluorescence epi-illumination, including a complete filter set for voltage-sensitive dye imaging with the new “blue dyes.” Together with the appropriate camera lenses (third party accessory, sold separately), this assembly can provide up to 100x increase in brightness relative to standard microscope lenses (see Ratzlaff and Grinvald, 1991 for details).

Set of filters for use in intrinsic imaging experiments.

Green
546, 30nm
Orange
605, 10nm
Red
630, 30nm
IR
700 bandpass 30nm
IR
810 bandpass 30nm

Other Filters

  • OD 3 neutral density filter
  • KG1 heat filter

Includes components pictured at left: lamp housing with tungsten-halogen lamp and integrated KG1 heat filter, bandpass filter holder, shutter, light guide adaptor, and double-ended liquid light guide with focusing lenses.

Two models of respirator are available, designed for the size of the subject animal. Different compression cylinder sizes are available to further adjust ventilation volume. These ventilators come with a start/stop option, which allows them to be remotely controlled by Imager 3001 systems.

Cat/Rabbit
Cat. 6125
Rodent
Cat. 7125

  • Tandem lens assembly: two 50 mm lenses (f 1.2), with back-to-back coupling ring and C-Mount adapter. For 1x magnification.
  • 135 mm lens (f 2.0), for 2.7x or 0.37x magnification (requires tandem lens assembly).
  • 2x tele-converter for increasing or decreasing magnification of tandem lens assembly by a factor of two (requires tandem lens assembly).

The Kepco JQE 15-25 (M) is a high-grade regulated power supply for use in stabilizing preparation illumination. It provides adjustable DC output up to 15 volts and 10 amps. Ripple is < 0.1% [/one_half] [one_half last="yes"]